Getting started

Typical requirement for setting up a complete screen of 96 experiments.

• 100 microliter pure protein @ 10 mg/ml
• Short meeting about expectations – what questions do you want to have answered.
• Data collection and data processing, structure solution, will be negotiated
• For initial crystallization plate setup there is a set price per crystal plate

Success is linked to prior chemical knowledge of the system of interest

• Is the protein monodisperse in solution (SEC)?
• Does it require co-factor or other special conditions? e.g FAD, ATP etc
• Does it contain glycans, or other post-translation modifications?
• Do primary structure include floppy regions such as a Tag, or intrinsically disordered regions (IDR’s)?