PhD student in Engineering feedback regulation for recombinant protein production

mandag 06 jul 20

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Frist 7. august 2020
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The department of Biotechnology and Biomedicine at the Technical University of Denmark invites applications for a position as a PhD student working on “Design and engineering of gene regulation systems in the budding yeast, Saccharomyces cerevisiae.”

The position is part of the FBM Initiative, which is funded by the Novo Nordisk Foundation. The FBM Initiative is a collaboration between the DTU departments of Chemical Engineering, Biotechnology and Biomedicine (DTU Bioengineering), and the Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain). It offers research-based education in Fermentation Based Biomanufacturing for Master- and PhD- students, and professionals.

This position will be hosted at DTU Bioengineering and DTU Biosustain and is one of seven PhD student positions available for the FBM PhD program this year.

This position will be placed in DTU Bioengineering, section of Synthetic Biology in the group of Fungal Systems Biology. The Synthetic Biology section has a strong focus in genetic engineering techniques in filamentous fungi as well as conventional and nonconventional yeasts. The section research spans synthetic biology, bioreactor cultivations for quantitative physiology, regulation engineering, protein production, and discovery of bioactive compounds. The section has a total of 45 staff, 9 of which are faculty.

Responsibilities and Tasks
Through the application of synthetic biology and state-of-the-art fermentation technology, the successful candidate will engineer novel feedback regulation for protein production in the budding yeast S. cerevisiae. Related to this, the following project aims will be pursued:

  • Design of DNA constructs to be used in the regulation of heterologous protein production
  • Implementation and testing of designed regulatory circuits in a model protein production strain of S. cerevisiae using micro-fermentations and controlled bioreactors at the 1 L scale.
  • Analysis and asessement of expression data for specific genes and genome-wide transcriptomics and mass spec proteomics.

The successful candidate will conduct their project in a collaboration between DTU Bioengineering, DTU Biosustain, and industrial partner Enduro Genetics.

Qualifications
Candidates should have a Master's degree in engineering or a similar degree with an academic level equivalent to the Master's degree in engineering.

We are looking for a candidate with a solid background in:

  • Experimental molecular biology
  • Genetic engineering and synthetic biology techniques 
  • Cell cultivation and fermentation technology
  • DNA sequence analysis and design of DNA constructs

We expect that you are an efficient team worker, have good communication skills, and have a critical approach to the formulation and testing of hypotheses. Also, a good sense of humor will be appreciated.

Approval and Enrolment
The scholarship for the PhD degree is subject to academic approval, and the candidate will be enrolled in one of the general degree programs at DTU. For information about our enrolment requirements and the general planning of the PhD study program, please see the DTU PhD Guide.

Assessment
The assessment of the applicants will be made by Christopher Workman and Michael Krogh Jensen and approved by an internal PhD assessment committee.

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and Appointment Terms
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union. The period of employment is 3 years.

You can read more about career paths at DTU here.

Further information
Further information may be obtained from Christopher Workman, cwor@dtu.dk, or +45 2365 2618.

You can read more about DTU Bioengineering at www.bioengineering.dtu.dk.

Application
Please submit your online application no later than 7 August 2020 (23:59 local time).

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

  • A letter motivating the application (cover letter)
  • Curriculum Vitae
  • Grade transcripts and BSc/MSc diploma
  • Excel sheet with translation of grades to the Danish grading system (see guidelines and Excel spreadsheet here)

Candidates may apply prior to ob­tai­ning their Master's degree but cannot begin before having received it.

All interested candidates irrespective of age, gender, race, disability, religion or ethnic background are encouraged to apply.

The Fermentation Based Biomanufacturing (FBM) Initiative was founded as a response to the biotech industry’s increasing demand for specialized expertise in the fermentation area. Cutting-edge research, covering all aspects from fundamental physiology of production organisms/cell factories, synthetic biology and metabolic engineering, to scale-up process technology and downstream processing, provides the basis for the specialized FBM Education. The educational activities are developed in close dialogue with industry partners.

The FBM Initiative at DTU educates top-candidates at MSc and PhD level, and provides research-based expertise for the biotechnological, chemical, and pharmaceutical fermentation based biomanufacturing industry.

The Department of Biotechnology and Biomedicine (DTU Bioengineering) conducts research, educates future bio-engineers, provides scientific advice and innovation within the areas of microbiology, biochemistry, biotechnology and biomedicine. The research at DTU Bioengineering is at the highest international level and focuses on the societal and scientific challenges within the field. Research is conducted within three main areas: Microbial ecology and physiology, Industrial biotechnology and cell factories, and Biomedicine and health. The department has extensive collaboration with national and international research units and industries. DTU Bioengineering has approx. 160 employees, of which 2/3 are scientific staff. The department is located at DTU Lyngby Campus.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 11,500 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. Our main campus is in Kgs. Lyngby north of Copenhagen and we have campuses in Roskilde and Ballerup and in Sisimiut in Greenland.