CUPP is a new approach for sequence analysis based on the hypothesis that conserved peptide patterns can be used to identify similarities between proteins.

CUPP performs automated clustering of proteins for formation of protein groups and can be used for fast annotation of enzyme protein family, subfamily and EC function of carbohydrate-active enzymes.

The CUPP clustering and CUPP prediction programs can be downloaded from the following link along with a CUPP library of CAZy families:

Download CUPP files here.

A new CUPP webserver for CAZyme annotation is available on the following link:

If you have any questions, please contact

When using the CUPP programs or results, please cite:

Barrett K, Lange L. Peptide-Based Classification and Functional Annotation of Carbohydrate-Active Enzymes by Conserved Unique Peptide Patterns (CUPP).
Biotechnol Biofuels. BioMed Central; 2019;1–21.